Irrespective of the different hydrodynamic diameters of Carbo

Fig. 4. (a) Scanning R 428 micrograph of the gut of an adult zebrafish (lateral cut) after 96 h of exposure to 100 mg/L of aged Carbo-Iron. The white areas in the lumen of the gut represent particles with Carbo-Iron-like morphology. Detection parameters: beam accelerating voltage (Acc. V.) = 25 kV, spot size (spot) = 5 nm, magnification (Magn.) = 1000 ×, detector = secondary electron detector, working distance (WD) = 10 mm. (b) EDX mapping of the same area detected the presence of iron in the particles and confirmed that they represent Carbo-Iron. The respective areas are highlighted as white clusters in the gut lumen.Figure optionsDownload full-size imageDownload as PowerPoint slide
3.4. Fish early life stage test
Fig. 5. (a) Overall survival (% of number of eggs at the start of exposure), (b) total length, and (c) individual fresh weight of zebrafish at the end of the fish early life stage test (34 days post fertilization). All embryos hatched successfully. Data represent means ± sd (n = 4) except for length and weight in the highest test concentration, where data are also presented excluding one replicate with a lower survival rate, which led to a faster growth (gray circles, n = 3). Co: control, Ccmc: control with the dispersant carboxymethyl cellulose (CMC, 20 mg/L); # indicates a significant difference to Co (two-sided Welch t-test, p < 0.05), the asterisk indicates a significant difference to Ccmc (ANOVA; two sided Dunnett test, p < 0.05).Figure optionsDownload full-size imageDownload as PowerPoint slide