From the data of LDH Fig a after grafted
For the APTT measurement, the platelet-poor AP20187 (PPP) was obtained by centrifuging the whole blood at 3000 rpm. Then 500 μL PPP was dropped onto the surface of the samples in a 24-well plate. The plate was incubated in a 37 °C for 15 min. And 100 μL actin-activated cephthaloplastin reagent were added into the above tube, followed by the addition of a 0.025 M CaCl2 solution after 3 min incubation at 37 °C.