The complexes obtained were performed elemental analyses Elemental
K2[Ni(CN)4]·H2O was prepared by mixing stoichiometric amounts nickel(II) chloride hexahydrate (1 mmol, NiCl2·6H2O = 0.238 g) in water (10 mL) with potassium cyanide (4 mmol, KCN = 0.260 g) in water (10 mL) solution. M[Ni(CN)4]·H2O was prepared by mixing together the water solution of 1 mmol (0.258 g) K2[Ni(CN)4]·H2O. To this AMG 487 K2[Ni(CN)4]·H2O solution, 1 mmol metal(II) chloride (MnCl2 = 0.126 g, FeCl2·4H2O = 0.199 g or CoCl2 = 0.130 g) dissolved in water (10 mL) were added with continuous stirring approximately for 4 h at 50 °C in a temperature-controlled bath. The compounds were filtered and washed with water and dried in open air. To Human Genome Project 1 mmol of M[Ni(CN)4]·H2O (M = 0.236 g for Mn(II), 0.236 g for Fe(II) and 0.240 g for Co(II)) water solution, 4 mmol (0.385 g) of etim dissolved in ethyl alcohol (10 mL) was added a few drops to resulting solution with continuous stirring approximately for 5 h at 60 °C in a temperature-controlled bath and then filtered, washed with water, ethanol, and ether, respectively, and dried at room temperature.