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The mechanical properties of products in enzymatically triggered reaction were affected by enzyme concentration (Guilbaud et al., 2013). Therefore, we investigated the role of enzyme amount in this G007-LK catalytic reaction, which revealed that the degradation of fenpropathrin was significantly increased with growing protein amount with the degradation rate peaking at 55.9% as 500 μL of extracts added (Fig. 4e). It is worth noting that the degradation rates showed no difference in treatments with extract volume exceeding 300 μL while the degradation of the treatments under 300 μL was dissatisfied, suggesting 300 μL of cell-free extracts was the equilibrium point of enzyme amount in fenpropathrin degradation.
Taken together, the tested factors could impact the enzymatic reaction in different degrees, which provided basic information and application skills of the cell-free extracts. We found that the cell-free extracts could function well under variable conditions, and it could be more effective with fenpropathrin at 25 to 100 mg·L− 1, pH value of 8, 30 °C, 30 min, and 300 μL of enzyme solution. Factors like residue concentrations and temperature changes in the environment are unpredictable and uncontrollable. Hence, it should be clearly declared that the parameters obtained here do not limit the utilization rage of the microbial extract.