Eleven seafood samples of fish shrimp shellfish as
For the acute exposure bioassay (single exposure OECD, 2013), a food solution containing 1.43 ng a.i./μL of thiamethoxam in diet C, corresponding to 1/10 of LC50, was prepared. On the 4th day of the bioassay, larvae were exposed to acute treatment (with 30 μL of food solution containing 1.43 ng a.i./μL, and on the 5th and 6th day they Saracatinib received only food without the insecticide. For subchronic bioassay (repeated oral exposure with less than 10% of dead – EPA, 2011), we use the same concentration of acute intoxication, divided into 3 consecutive days, which is equivalent to 0.47 ng a.i/μL per day. Larvae were daily fed on the 4th, 5th, and 6th days with 30, 40 and 50 μL of contaminated food solution. The control group received an uncontaminated diet B cells consisted only of diet C. Exposure of larvae to thiamethoxam through the food was perfectly controlled. During the evaluations, larval mortality and atypical development were assessed (i.e., larval positioning and changes in the progression of larval instars). For each exposure condition, 3 replicates of 12 larvae were used.