Relative protein expression levels of indicated
Cell viability assay
The A66 seeded at a density of 1 × 105 cells/well into 96-well plates were cultured with 100 μL of culture medium per well for 24 hours and with 90 μL of culture medium plus 10 μL CCK-8 reagents per well for 1 hour. The optical density at 490 nm (OD490 nm) for each well was determined by an enzyme immunoassay analyzer.
Annexin V-FITC/propidium iodide double-staining
After irradiation, the cells were double-stained with Annexin V-FITC and propidium iodide, according to the manufacturer\'s instruction (Solarbio, Bei Jin, China), and then analyzed with a dual laser flow cytometer (Becton Dickinson, San Jose, CA, USA). The extent of apoptosis was estimated using ModFit LT software (Verity Software House, Topsham, ME, USA).
Wound healing assay
Cells were trypsinized and seeded in 6-well plates, and each well was divided into a 2 × 3 grid after the cells reached confluence. An artificial homogenous wound was created by scratching the cell monolayer with a 1-mL pipette tip and exposing cells to low-dose irradiation. The culture medium was replaced with FBS-free medium in the next round of cultivation. Microscopic images of the same area were captured immediately after a wound was inflicted as well as at 24 hours. Cell migration rate was calculated using the following equation: