Fig Scanning electron micrographs of control a
2.2. Test electrolytes solutions
As described in Table 1, two electrolyte solutions were utilized in this study. The normal electrolyte solution consisted of alpha minimum essential medium (AMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Atlanta Biologicals) and 1% penicillin/streptomycin (Gibco). The AMEM solution contained sodium bicarbonate (NaHCO3), which in combination with the 5% CO2 INNO-206 of the incubator, contributed to maintaining the pH around 7.4. The normal electrolyte solution was used to model the biological environment in the absence of an inflammatory reaction. A modified electrolyte solution was composed of the normal electrolyte solution with the addition of 150 mM (5400 ppm) H2O2 (J.T. Baker) and titrated to pH = 5 using HCL (J.T. Baker). These solution modifications have previously been described and utilized  and  to simulate the biological environment in the presence of an inflammatory reaction.
Scanning electron micrographs of control (a, c) and inflammatory (b, d) AZ91 ...