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Statistical evaluation was carried out by Student's two tailed t-test, and P value < 0.05 was considered to be statistically significant.2.13. Nucleotide Sequence Accession NumbersThe Interleukin-11 receptor accession numbers of 16SrRNA and 5��coding region of bap sequences (amplified from eDNA) was "type":"entrez-nucleotide","attrs":"text":"HM992508","term_id":"302316221","term_text":"HM992508"HM992508 and "type":"entrez-nucleotide","attrs":"text":"HM765514","term_id":"303306205","term_text":"HM765514"HM765514, respectively.3. Results3.1. Presence of eDNA in Extracellular Growth MediumTo check the presence of DNA in the extracellular growth medium of A. baumannii AIIMS 7, eDNA was purified by isopropanol precipitation at ice-cold temperature. eDNA was found to be present along the temporal scale of A.
baumannii AIIMS seven growth as much as 96 hrs and showed a pattern as proven in graph (Figure one(b)). The pattern of eDNA (Figure 1(b)) showed practically equivalent concentrations of eDNA while in the early development phase (up to 36 hours), a fall inside the mid-stationary phase (48 hour), followed by regular concentrations within the late growth phases (60 to 96 hours). inhibitor AR-12 Correlation of development curve (Figure one(a)) and concentration of purified eDNA showed that the prevalence of eDNA was pretty much consistent. Concentrations of eDNA were comparable to earlier findings in Pseudomonas aeruginosa . Figure one(a) Growth curve of Acinetobacter baumannii AIIMS 7. (b) Pattern of eDNA no release in temporal scale of the. baumannii AIIMS 7 growth. Graph representing concentrations of eDNA purified from cell-free supernatant of the. baumannii AIIMS seven at respective time ...3.two. Membrane Vesicles Present in Extracellular Growth Medium Contained DNAMembrane vesicles had been purified from cell-free supernatant (early development phase) and characterized using electron microscopy to find out their dimension and morphology.